In silico explorations of bacterial mercuric reductase as an ecofriendly bioremediator for noxious mercuric intoxications
Mercury is a major pollutant in the environment due to its high concentration in the soil. In this study, a mercuric reductase was extracted from Pseudomonas aeruginosa. The sequence of the enzyme was retrieved from the literature and structural homologs were identified. The protein bonded with Mercuric compounds and their interaction was briefly studied. Autodock Vina was used to perform a molecular docking with the target protein. Results showed that the sequence consists of most of the random coil 44.74% followed by α-helix and B-turns. Moreover, the protein was predicted to have a FAD/NAD(P)-binding domain. The virulence factor prediction using different approaches of Virulentpred and VICMpred suggested that P00392 is non-toxic. Next, the mutational analyses were performed to predict the active site residues in the resulting models and to determine mutants. The results show that the enzyme is involved in the bioremediation of mercury by using in-silico techniques. Finally, molecular docking studies were conducted on the best-selected model to find the active site residues and to generate a pattern of interaction to understand the mode of action of the substrate and its catalytic activity which refers to the binding with mercury.
Copyright (c) 2023 Muhammad Naveed, Muhammad Aqib Shabbir, Tariq Aziz, Ayesha Saleem, Rida Naveed, Ayaz Ali Khan, Taqweem Ul Haq, Metab Alharbi, Abdulrahman Alshammari, Abdullah F. Alasmari
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