MicroRNA-1298-3p induces tumor-suppressive effects in  human cervical cancer cells via post-transcriptional  suppression of ONECUT2

Kun Li; Min Zhou; Yan Zhang

doi:10.18388/abp.2020_5919

MicroRNA-1298-3p induces tumor-suppressive effects in human cervical cancer cells via post-transcriptional suppression of ONECUT2

Kun Li Department of gynaecology and obstetrics, The Third Affiliated Hospital of Shandong First Medical University (Affiliated Hospital of Shandong Academy of Medical Sciences), Jinan City, Shandong Province, 25003,1 China
Min Zhou Department of gynaecology and obstetrics, Jinan Seventh People’s Hospital, Jinan City, Shandong Province, 250101, China
Yan Zhang Department of gynaecology and obstetrics, The Third Affiliated Hospital of Shandong First Medical University (Affiliated Hospital of Shandong Academy of Medical Sciences), Jinan City, Shandong Province, 25003,1 China

DOI: https://doi.org/10.18388/abp.2020_5919

Abstract

Recent studies have revealed the negative regulatory role of microRNA-1298-3p (miR-1298-3p) in human carcinogenesis. However, the role of miR-1298-3p is yet to be studied in cervical cancer. The present study showed significant (P=0.03) downregulation of miR-1298-3p in human cervical cancer cell lines. Overexpression of miR-1298-3p significantly (P=0.02) suppressed the proliferation of the cervical cancer cells via induction of apoptosis. The percentage of early and late apoptotic cervical cancer cells increased from 3.33% to 43.37% upon miR-1298-3p overexpression. Additionally, expression of Bax was increased and that of Bcl-2 was decreased in miR-1298-3p overexpressing cervical cancer cells. Overexpression of miR-1298-3p could also suppress migration and invasion of the cervical cancer cells. In vivo study showed that miR-1298-3p overexpression significantly (P=0.02) inhibited the xenografted tumor-growth via induction of apoptosis. In silico analysis and subsequent in vitro assays revealed that miR-1298-3p exerts its effects by targeting the expression of ONECUT2. While silencing of ONECUT2 could suppress the proliferation of cervical cancer cells, its overexpression could nullify the tumor-suppressive effects of miR-1298-3p. Collectively, the results revealed the tumor-suppressive role of miR-1298-3p in cervical cancer .and thus, indicative of its future therapeutic utility.

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Published

2022-12-12

Issue

Vol. 69 No. 4 (2022)

Section

Articles

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