Use of transcriptional slippage for diverse gene expression

Dawid  Koscielniak; Ewelina  Sobisz; Ewa Wons; Marian Sęktas

doi:10.18388/abp.2020_5736

Use of transcriptional slippage for diverse gene expression

Dawid Koscielniak Department of Microbiology, Faculty of Biology, University of Gdansk, Gdańsk, Poland
Ewelina Sobisz Department of Microbiology, Faculty of Biology, University of Gdansk, Gdańsk, Poland
Ewa Wons Department of Microbiology, Faculty of Biology, University of Gdansk, Gdańsk, Poland
Marian Sęktas Department of Microbiology, Faculty of Biology, University of Gdansk, Gdańsk, Poland

DOI: https://doi.org/10.18388/abp.2020_5736

Abstract

We present here an alternative for two-promoter systems ensuring highly diverse expression of several genes from a single promoter. This approach assumes an introduction of a deletion mutation into an A/T homopolymeric run in a gene’s proximal part, and employs the transcriptional slippage mechanism for insertion-dependent reinstatement of the proper reading frame by the T7 RNA polymerase.

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Published

2021-08-26

Issue

Vol. 68 No. 3 (2021): Issue dedicated to Professor Wacław Tadeusz Szybalski on the 100th anniversary of his birth

Section

Articles

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