Use of transcriptional slippage for diverse gene expression
We present here an alternative for two-promoter systems ensuring highly diverse expression of several genes from a single promoter. This approach assumes an introduction of a deletion mutation into an A/T homopolymeric run in a gene’s proximal part, and employs the transcriptional slippage mechanism for insertion-dependent reinstatement of the proper reading frame by the T7 RNA polymerase.
Copyright (c) 2021 Marian Sęktas, Dawid Koscielniak, Ewelina Sobisz, Ewa Wons
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