A rapid method for protein staining in polyacrylamide gels using water saturated with chloroform

Paweł Piotr Pieta; Ewa Burchacka; Aleksandra Śliwa; Anna Szczerba

doi:10.18388/abp.2020_5678

A rapid method for protein staining in polyacrylamide gels using water saturated with chloroform

Paweł Piotr Pieta Department of Bionic and Experimental Medical Biology, Poznan University of Medical Sciences, Poznań, Poland
Ewa Burchacka Department of Medical and Organic Chemistry, and Microbiology, Faculty od of Chemistry, Wroclaw University of Science and Technology, Wrocław, Poland
Aleksandra Śliwa Cell Biology Department, Poznan University of Medical Sciences, Poznań, Poland
Anna Szczerba Cell Biology Department, Poznan University of Medical Sciences, Poznań, Poland

DOI: https://doi.org/10.18388/abp.2020_5678

Abstract

Polyacrylamide gel electrophoresis, followed by an appropriate staining, is a popular and useful analytical procedure for protein identification and characterization. The aim of this study was to develop a method for protein visualization in polyacrylamide gels that would be alternative to Coomassie blue or silver staining. The proposed method is simple, fast and inexpensive. The optimized protocol for protein staining and visualization takes as little as 6 minutes and utilizes deionized water and chloroform. Fluorescence of proteins is induced by UV light and can be detected with a standard transilluminator.

pdf
html

Published

2021-05-11

Issue

Vol. 68 No. 2 (2021)

Section

Articles

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Acta Biochimica Polonica is an open access quarterly and publishes four issues a year. All contents are distributed under the Creative Commons Attribution-ShareAlike 4.0 International (CC BY-SA 4.0) license. Everybody may use the content following terms: Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made, ShareAlike — If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original. There are no additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.