miREIA - an immunoassay method in assessment of microRNA levels in tumor tissue-pilot study. The impact of miR-93-5p, miR-142-5p and IFNγ on PD-L1 level in colorectal cancer
Colorectal cancer (CRC) is the second and third most common cancer in females and males, respectively. The PD-L1/PD-1 immune checkpoint is an important source of immunosuppression in the tumor microenvironment and is associated with IFNγ. Recent studies revealed that a significant number of tumor-suppressive miRNAs can regulate the expression of PD-L1. The objective quantification of selected microRNAs using the miREIA method in CRC tissue was performed. We investigated the role of miR-93-5p and miR-142-5p expression and the levels of IFNγ in regulating the expression of PD-L1 in tumor and margin tissues of CRC in relation to the histological grade, TNM classification, and tumor localization. 37 samples of tumor and margin tissues from CRC patients were evaluated. MiR-93-5p and miR-142-5p levels were measured using a novel method for the quantitative measurement of human microRNA (miREIA). The concentrations of PD-L1 and IFNγ were determined using ELISA kits. We found higher concentrations of miR-93-5p, PD-L1, and IFNγ in tumor samples compared to the tumor margin samples. A significant correlation was found between PD-L1 and IFNγ levels in tumor and margin specimens; between miR-142-5p and miR-93-5p levels in tumor and margin specimens. A higher level of miR-93-5p was found in tumor margin tissues on the left side of the colon. Patients with distant metastases were characterized by higher miR-93-5p concentration compared to patients without metastases. CRC is an important source of PD-L1, IFNγ, and miR-93-5p expression. Understanding the mechanisms underlying intratumoral PD-L1 expression may create new opportunities for targeted immunotherapy of colorectal cancer.
Copyright (c) 2021 Miriam Dawidowicz, Agnieszka Kula, Sylwia Mielcarska, Paweł Kiczmer, Karolina Gołąbek, Zofia Ostrowska, Dariusz Waniczek, Elżbieta Świętochowska
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