Resveratrol enhances apoptosis induced by the heterocyclic aromatic amines in p53-wt LoVo cells, but not in p53-deficient HaCaT cells

Katarzyna   Kuryłowicz; Agnieszka Cierniak; Ewelina Madej; Łukasz Skalniak; Agnieszka Wolnicka-Głubisz

doi:10.18388/abp.2020_5477

Resveratrol enhances apoptosis induced by the heterocyclic aromatic amines in p53-wt LoVo cells, but not in p53-deficient HaCaT cells

Katarzyna Kuryłowicz Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland https://orcid.org/0000-0003-1456-8097
Agnieszka Cierniak Department of Medicine and Health Sciences, Andrzej Frycz Modrzewski Krakow, University, Kraków, Poland https://orcid.org/0000-0001-6537-2954
Ewelina Madej Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland https://orcid.org/0000-0002-0729-0245
Łukasz Skalniak Faculty of Chemistry, Jagiellonian University, Kraków, Poland https://orcid.org/0000-0002-6707-6697
Agnieszka Wolnicka-Głubisz Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland https://orcid.org/0000-0003-3070-6349

DOI: https://doi.org/10.18388/abp.2020_5477

Abstract

In the present study, we investigated the influence of resveratrol on PhIP treated human colon cancer cells and compared the effect to HaCaT cells considered as normal, human keratinocytes. Our results show that resveratrol decreases DNA damage in both cell types, it increases the sensitivity of LoVo cells to apoptosis and has no effect on PhIP-treated HaCaT cells. We confirm that PhIP-induced apoptosis is p53 and caspase 3/7 dependent. Interestingly, normal cells such as HaCaT, which lack functional p53 are more resistant to PhIP treatment.

Author Biographies

Agnieszka Cierniak, Department of Medicine and Health Sciences, Andrzej Frycz Modrzewski Krakow, University, Kraków, Poland

Department of Medicine and Health Sciences, Andrzej Frycz Modrzewski Krakow University, Herlinga-Grudzińskiego 1, 30-705 Krakow, Poland

Ewelina Madej, Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland

Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland

Łukasz Skalniak, Faculty of Chemistry, Jagiellonian University, Kraków, Poland

Faculty of Chemistry, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland

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Published

2020-10-27

Issue

Vol. 67 No. 4 (2020)

Section

Articles

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