Comparing the effects of different exogenous hormone combinations on seed-derived callus induction in Nicotiana tabacum

Jakub Lang; Jędrzej Pankowski; Piotr Grabarz; Bartosz Pluciński; Paweł Jedynak

doi:10.18388/abp.2020_5456

Comparing the effects of different exogenous hormone combinations on seed-derived callus induction in Nicotiana tabacum

Jakub Lang Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland https://orcid.org/0000-0003-0045-7740
Jędrzej Pankowski Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland https://orcid.org/0000-0003-3968-1790
Piotr Grabarz Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland
Bartosz Pluciński Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland https://orcid.org/0000-0002-5193-4562
Paweł Jedynak Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland https://orcid.org/0000-0001-7686-3927

DOI: https://doi.org/10.18388/abp.2020_5456

Abstract

Callus from Nicotiana tabacum is used as a model in plant developmental research. We tested several phytohormone (Indoleacetic acid – IAA; 2,4-Dichlorophenoxyacetic acid – 2,4-D; kinetin – KIN; 6-Benzylaminopurine – BAP) combinations to compare different approaches to callus induction directly from the seeds of Nicotiana tabacum. Callus formation was observed up to 4 weeks after sowing and the most effective were 0.5 mg/L of 2,4-D with 0.25 mg/L of BAP and 2 mg/L 2,4-D with 1 mg/L of BAP. The calli were green, photosynthetically active and after 6 weeks of growth, no stress symptoms (estimated on the basis of fluorescence of chlorophyll a in photosystem II) were noticed.

Author Biography

Paweł Jedynak, Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland

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Published

2020-11-27

Issue

Vol. 67 No. 4 (2020)

Section

Articles

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