A novel method for rapid and quantitative detection of bisphenol A in urine

  • Aleksandra Rutkowska Gdańsk Medical University https://orcid.org/0000-0002-7157-9967
  • Aleksandra Olsson DetoxED Ltd., Gdansk, Poland https://orcid.org/0000-0002-2759-5126
  • Jacek Namieśnik Department of Analytical Chemistry, Faculty of Chemistry, Gdańsk University of Technology, Gdańsk, Poland
  • Andrzej Milewicz Faculty of Natural Sciences and Technology, Karkonosze College in Jelenia Góra, Jelenia Góra, Poland
  • Jan Krzysztof Ludwicki Department of Toxicology and Health Risk Assessment, National Institute of Public Health – National Institute of Hygiene, Warsaw, Poland https://orcid.org/0000-0002-4399-1751
  • Paweł Struciński Department of Toxicology and Health Risk Assessment, National Institute of Public Health – National Institute of Hygiene, Warsaw, Poland https://orcid.org/0000-0001-6023-283X
  • Szymon Graczyk DetoxED Ltd., Gdansk, Poland

Abstract

Bisphenol A (BPA) is classified as an endocrine disruptor (ED) and it can interact with variety of hormone receptors leading to hormonal disruption and increased risk of various adverse health effects. Reducing human exposure to BPA is one of the main challenges of public health, as it is constantly present in daily life. A low-cost and commonly applied method to enable determination of BPA in the patient's body has yet to be developed. Currently available techniques are expensive, time-consuming, and require access to highly equipped analytical chemistry laboratories. Here we describe a fast and cheap engineered lateral flow assay of our design, to detect of BPA in urine samples. The technology not only provides an opportunity to perform rapid medical diagnostics without the need for an access to the central laboratory but also a means for self-diagnosis by the patient. The addition of β-glucuronidase improves the sensitivity of detection as it releases the free BPA from glucuronide complexes in urine. This invention may become a demonstrated analytical means for lowering human exposure to BPA and probably also to other EDs and consequently, may be useful in decrease of the risk for several lifestyle diseases.

Author Biography

Jacek Namieśnik, Department of Analytical Chemistry, Faculty of Chemistry, Gdańsk University of Technology, Gdańsk, Poland

†Deceased 14 April 2019

Published
2020-07-30
Section
Articles