Molecular characterization of a multidrug-resistant/pandrug-resistant nosocomial polymicrobial infection with Klebsiella pneumoniae, Providencia rettgeri, and Acinetobacter baumannii from Rural Maharashtra, India

Dilip D. Karad; Yogesh Somani; Hemant Khande; Bipin Yadav; Arun S. Kharat

doi:10.18388/abp.2020_5239

Molecular characterization of a multidrug-resistant/pandrug-resistant nosocomial polymicrobial infection with Klebsiella pneumoniae, Providencia rettgeri, and Acinetobacter baumannii from Rural Maharashtra, India

Dilip D. Karad Department of Microbiology, Shri Shivaji Mahavidyalaya, Barshi, Solapur, Maharashtra, 413411, India
Yogesh Somani Dr. Yogesh Somani Hospital, Barshi, Solapur, Maharashtra, 413401, India
Hemant Khande Wockhardt Research Centre, Aurangabad, Maharashtra, 431210, India https://orcid.org/0000-0001-7821-8534
Bipin Yadav Laboratory of Applied Microbiology, School of Life Sciences, Jawaharlal Nehru University, New Mehrauli Road, New Delhi, 110067, India https://orcid.org/0000-0002-0333-9582
Arun S. Kharat Laboratory of Applied Microbiology, School of Life Sciences, Jawaharlal Nehru University, New Mehrauli Road, New Delhi, 110067, India https://orcid.org/0000-0002-2571-4398

DOI: https://doi.org/10.18388/abp.2020_5239

Abstract

The emergence of resistance against commonly used antibiotics has become a serious global concern. The rapid development of antibiotic resistance exhibited by Enterobacteriaceae has caused an increasing concern regarding untreatable bacterial infections. Here, we isolated four pathogens from a geriatric female patient who was hospitalized for a month with ventilator-associated pneumonia (VAP) and fever. The organisms isolated from the tracheal aspirates and urine included Klebsiella pneumoniae, pandrug-resistant Providencia rettgeri, and Acinetobacter baumannii. Resistome analysis indicated that the bacterial isolates from the polymicrobial infection were multiple-drug resitnat and pandrug resistant clones. Molecular characterization revealed presence of blaTEM-1 in K. pneumonaie, P. rettgeri and A. baumannii. The blaTEM-1 and blaNDM-1 genes were present in P. rettgeri and A. baumannii, whereas the blaTEM-1, blaNDM-1 and blaOXA-23 traits were present in A. baumannii isolates. The patient has died due to the unavailability of effective antimicrobial treatment for this drug-resistant polymicrobial infection.

Author Biographies

Dilip D. Karad, Department of Microbiology, Shri Shivaji Mahavidyalaya, Barshi, Solapur, Maharashtra, 413411, India

Professor in Microbiology

Arun S. Kharat, Laboratory of Applied Microbiology, School of Life Sciences, Jawaharlal Nehru University, New Mehrauli Road, New Delhi, 110067, India

School of Life Science

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Published

2020-07-31

Issue

Vol. 67 No. 3 (2020)

Section

Articles

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