MiR-139-5p alleviates neural cell apoptosis induced by spinal cord injury through targeting TRAF3

Ziying Zhang; Lifang Shen; Yingying Yan

doi:10.18388/abp.2020_5198

MiR-139-5p alleviates neural cell apoptosis induced by spinal cord injury through targeting TRAF3

Ziying Zhang Department of Rehabilitation Medicine, The Fifth Affiliated Hospital of Zhengzhou University, Z, Zhengzhou, Henan Province, 450052, PR China https://orcid.org/0000-0002-7328-2091
Lifang Shen Department of Rehabilitation Medicine, The Fifth Affiliated Hospital of Zhengzhou University, Z, Zhengzhou, Henan Province, 450052, PR China https://orcid.org/0000-0002-1990-905X
Yingying Yan Department of Rehabilitation Medicine, The Fifth Affiliated Hospital of Zhengzhou University, Z, Zhengzhou, Henan Province, 450052, PR China https://orcid.org/0000-0002-9218-1851

DOI: https://doi.org/10.18388/abp.2020_5198

Abstract

Spinal cord injury (SCI) is a neurological trauma that causes loss of locomotor function and sensory deficit. Previous studies showed that miRNAs play a crucial role in SCI. This study further evaluated the potential role of miR-139-5p in the neural cell apoptosis after SCI in rats. A rat SCI model was successfully established and miR-139-5p expression level in SCI rats was down-regulated compared to the sham group (sham operation group) determined by qRT-PCR. MiR-139-5p overexpression via administration with miR-139-5p agomir improved locomotor functional recovery, attenuated allodynia and hyperalgesia and alleviated neural cell apoptosis in SCI rats. In addition, TRAF3 (TNF receptor-associated factor 3 ) was identified to be a target of miR-139-5p by searching the proposed target genes in TargetScan 7.1 database. Co-transfection of miR-139-5p agomir and adenovirus of TRAF3 plasmids significantly improved functional recovery and alleviated neural cell apoptosis. Therefore, TRAF3 mediated the anti-apoptosis effect of miR-139-5p in SCI rats and miR-139-5p could be a promising candidate for SCI therapy by alleviating neural cell apoptosis through targeting TRAF3.

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Published

2020-08-27

Issue

Vol. 67 No. 3 (2020)

Section

Articles

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