Hydrogen sulfide formation in experimental model of acute pancreatitis

Patrycja Bronowicka-Adamska; Tomasz Hutsch; Marta Gawryś-Kopczyńska; Klaudia Maksymiuk; Maria Wróbel

doi:10.18388/abp.2019_2900

Hydrogen sulfide formation in experimental model of acute pancreatitis

Patrycja Bronowicka-Adamska Chair of Medical Biochemistry, Jagiellonian University, Collegium Modicum, Kraków, Poland
Tomasz Hutsch Department of Physiology and Experimental Pathophysiology, Medical University of Warsaw
Marta Gawryś-Kopczyńska Department of Physiology and Experimental Pathophysiology, Medical University of Warsaw
Klaudia Maksymiuk Department of Physiology and Experimental Pathophysiology, Medical University of Warsaw, Warsaw, Poland
Maria Wróbel Chair of Medical Biochemistry, Jagiellonian University, Collegium Modicum, Kraków, Poland https://orcid.org/0000-0001-7919-2778

DOI: https://doi.org/10.18388/abp.2019_2900

Abstract

Acute pancreatitis (AP) is a disease defined as acute or chronic inflammatory process of the pancreas characterized by premature activation of digestive enzymes within the pancreatic acinar cells and causing pancreatic auto-digestion. In mammalian tissues, H2S is synthesized endogenously from L-cysteine in regulated enzymatic pathways catalyzed by pyridoxal phosphate-dependent enzymes: cystathionine beta-synthase (CBS), gamma-cystathionase (CTH) and cysteine aminotransferase (CAT) coupled with 3-mercaptopyruvate sulfurtransferase (MPST). In the mitochondria, hydrogen sulfide is oxidized to sulfite, which is then converted to thiosulfate (a sulfane sulfur-containing compound) by thiosulfate sulfurtransferase (rhodanese; TST). Activity and expression of CBS, CTH, MPST, and TST have been determined in vivo in pancreas of the control rats, rats with acute pancreatitis and a sham group. Levels of low-molecular sulfur compounds, such as the reduced and oxidized glutathione, cysteine, cystine and cystathionine, were also determined. This study revealed a significant role of MPST in H2S metabolism in the pancreas. Stress caused by the surgery (sham group) and AP cause a decrease in H2S production associated with a decrease in MPST activity and expression. Markedly higher level of cysteine in the AP pancreas may be caused by a reduced rate of cysteine consumption in a reaction catalyzed by MPST, but it can also be a sign of proteolytic processes occurring in the changed tissue.

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Published

2019-12-27

Issue

Vol. 66 No. 4 (2019): Part I and Part II - FEBS

Section

FEBS 2019

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