Role of orf73 in the development of lambdoid bacteriophages during infection of the Escherichia coli host
Shiga toxin-producing Escherichia coli (STEC) is a group of pathogenic strains responsible for human infections that result in bloody diarrhea and hemorrhagic colitis, often with severe complications. The main virulence factors of STEC are Shiga toxins encoded by stx genes located in genomes of Shiga toxin-converting bacteriophages (Stx phages). These bacterial viruses are clustered in the lambdoid bacteriophages family represented by phage λ. Here, we report that expression of orf73 from the exo-xis region of the phage genome promotes the lysogenic pathway of development of λ and Φ24B phages. We demonstrated that the mutant phages with deletions of orf73 revealed higher burst size during the lytic cycle. Moreover, survival rates of E. coli infected with mutant bacteriophages were lower relative to wild-type viruses. Additionally, orf73 deletion negatively influenced the lysogenization process of E. coli host cells. We conclude that orf73 plays an important biological role in the development of lambdoid viruses, and probably it is involved in the network of molecular mechanism of the lysis-vs.-lysogenization decision.
Acta Biochimica Polonica is an OpenAccess quarterly and publishes four issues a year. All contents are distributed under the Creative Commons Attribution-ShareAlike 4.0 International (CC BY 4.0) license. Everybody may use the content following terms: Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
Copyright for all published papers © stays with the authors.
Copyright for the journal: © Polish Biochemical Society.