Identification of evolutionary conserved DNA sequence and corresponding S21 ribosomal protein region for diagnostic purposes of all Borrelia spirochetes
It is still under investigation, whether all Borrelia sp. causing Lyme borreliosis and other diseases are already identified and properly classified as human pathogens. For this reason, it is of great importance to develop a diagnostic ELISA test that detects all Borrelia sp. The aim of this study was to identify conserved DNA and protein regions present in all currently known Borrelia sp. In experimental studies 31 available Borrelia sp. genomes were aligned and screened for the presence of evolutionary conserved regions. As a result of bioinformatics analysis, one evolutionally conserved DNA region encoding a core fragment of the S21 ribosomal protein was identified. Both a couple of genus-specific PCR primers and the S21 protein B-cell epitope were designed for prospective diagnostic purposes.
Barstad B, Quarsten H, Tveitnes D, Noraas S, Ask IS, Saeed M, Bosse F, Vigemyr G, Huber I, Øymar K (2018) Direct Molecular Detection and Genotyping of Borrelia burgdorferi Sensu Lato in Cerebrospinal Fluid of Children with Lyme Neuroborreliosis. J Clin Microbiol 56(5). pii: e01868-17. doi: 10.1128/JCM.01868-17.
Bieri P, Leibundgut M, Saurer M, Boehringer D, Ban N (2017) The complete structure of the chloroplast 70S ribosome in complex with translation factor pY. EMBO J 36:475-486. doi: 10.15252/embj.201695959.
Cutler SJ, Ruzic-Sabljic E, Potkonjak A (2017) Emerging borreliae - Expanding beyond Lyme borreliosis. Mol Cell Probes 31:22-27. doi:10.1016/j.mcp.2016.08.003.
Jahfari S, Krawczyk A, Coipan EC, Fonville M, Hovius JW, Sprong H, Takumi K (2017) Enzootic origins for clinical manifestations of Lyme borreliosis. Infect Genet Evol 49:48-54. doi: 10.1016/j.meegid.2016.12.030.
Katoh K, Misawa K, Kuma K, Miyata T (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Res 15:3059-3066.
Källberg M, Wang H, Wang S, Peng J, Wang Z, Lu H, Xu J (2012) Template-based protein structure modeling using the RaptorX web server. Nat Protoc 7:1511-1522. doi:10.1038/nprot.2012.085.
Larsen JE, Lund O, Nielsen M (2006) Improved method for predicting linear B-cell epitopes. Immunome Res 2:2. doi:10.1186/1745-7580-2-2.
Rudenko N, Golovchenko M, Růzek D, Piskunova N, Mallátová N, Grubhoffer L (2008) Detection of Borrelia bissettii in cardiac valve tissue of a patient with endocarditis and aortic valve stenosis in the Czech Republic. J Clin Microbiol 46(10):3540–3543. doi:10.1128/JCM.01032-08.
Schneider BS, Schriefer ME, Dietrich G, Dolan MC, Morshed MG, Zeidner NS (2008) Borrelia bissettii isolates induce pathology in a murine model of disease. Vector-Borne Zoonotic Dis 8(5):623–633. doi:10.1089/vbz.2007.0251.
Sillanpää H, Skogman BH, Sarvas H, Seppälä IJ, Lahdenne P. (2014) Antibodies to decorin-binding protein B (DbpB) in the diagnosis of Lyme neuroborreliosis in children. Int J Infect Dis 28:160-163. doi: 10.1016/j.ijid.2014.07.006.
Stanek G, Reiter M (2011) The expanding Lyme Borrelia complex - clinical significance of genomic species. Clin Microbiol Infect 17(4):487–493. doi:10.1111/j.1469-0691.2011.03492.x.
Williams SK, Weiner ZP, Gilmore RD. (2018) Human neuroglial cells internalize Borrelia burgdorferi by coiling phagocytosis mediated by Daam1. PLoS One 13(5):e0197413. doi: 10.1371/journal.pone.0197413.
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