The inhibitory effects of rosmarinic acid on catabolism induced by IL-1β in rat chondrocyte

  • Zhong-Nan Hu Department of Orthopedics Surgery, The Children’s Hospital of Medical College, Zhejiang University, Hangzhou, People's Republic of China.
  • Li-Juan Huang Hemodialysis center, Division of Nursing, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, People's Republic of China
  • Wei-Ping Chen Department of Orthopedics Surgery, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, People’s Republic of China


The effects of rosmarinic acid (RosA) on osteoarthritis (OA) was investigated in rat chondrocytes. Chondrocytes were isolated from rat cartilage, incubated with RosA in the presence of interleukin-1beta (IL-1β) (10 ng/ml). The production of IL-6, as well as the mRNA level of aggrecan (ACAN) and collagen 2 (COL2), were assessed. The gene and protein expression of A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)-4, ADAMTS-5 were also measured. RosA inhibited the production of IL-6 as well as the gene and protein expression of ADAMTS-4 and ADAMTS-5, in addition, RosA abolished IL-1β-induced inhibition of ACAN and COL2 gene expression. Our results suggest that RosA can inhibit extracellular matrix (ECM) degradation in OA, thus, RosA may be a possible agent in the treatment of OA.


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