In silico analysis of different signal peptides to discover a panel of appropriate signal peptides for secretory production of Interferon-beta 1b in Escherichia coli

  • Shahrokh Ghovvati University of Guilan,
  • Zahra Pezeshkian University of Guilan,
  • Seyed Ziaeddin Mirhoseini University of Guilan,

Abstract

Signal peptides (SPs) are one of the most important factors for suitable secretion of the recombinant  heterologous proteins in Escherichia coli (E. coli). The objective of this study was to identify a panel of signal peptides (among the 90 biologically active SPs) required for the secretory production of interferon-beta 1b (IFN-beta 1b) recombinant protein into the periplasmic space of E. coli host. In the initial step, after predicting the accurate locations of the cleavage sites of signal peptides and their discrimination scores using SignalP 4.1 server, 31 SPs were eliminated from further analysis because their discrimination scores were less than 0.5 or their cleavage sites were inappropriately located. Therefore, only 59 SPs could be theoretically applied to secrete IFN-beta 1b into the periplasmic space of E. coli. The physico-chemical and the solubility properties, which are necessary parameters for selecting appropriate SPs, were predicted using ProtParam and SOLpro servers using the 59 remaining signal peptides. The final subcellular localization of IFN-beta 1b in combination with different SPs was predicted using ProtComB server. Consequently, according to the ranking of 59  confirmed SPs, the obtained results revealed that SPs Flagellar P-ring protein (flgI), Glucan1,3-beta-glucosidase I/II (EXG1) and outer membrane protein C (OmpC) were theoretically the most potentand desirable SPs for secretion of recombinant IFN-beta 1b into the periplasmic space of E. coli. For further studies in the future, the experimental investigations on the obtained results will be considered.

Author Biography

Shahrokh Ghovvati, University of Guilan,
Department of Biotechnology

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Published
2018-10-31
Section
Articles