Determination of norflurazon concentration in wheat leaves using a modified QuEChERS method

  • Kamil F. Trzebuniak
  • Beata Mysliwa-Kurdziel Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University
Keywords: norflurazon, QuEChERS, Wheat, Triticum aestivum, HPLC-UV


The aim of this analytical study was to develop and validate an easy-to-use method for measuring the actual level of norflurazon that accumulates in leaves. We amended the QuEChERS method, i.e. Quick, Easy, Cheap, Effective, Rugged, and Safe, which is widely used for pesticide and herbicide analysis in food, and usually combined with HPLC-MS detection. We adapted this method for the detection of norflurazon in leaves or leaf fragments
and proposed a useful modification using of HPLC-UV detection. Reproducible retention times of 3.11±0.04 min, precision (RSD<8.0%), LOQ=315 ng∙mL-1 and linearity (R2=0.99874) were achieved.


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