Berberine from Argemone mexicana L exhibits broadspectrum antibacterial activity

  • Nilesh V More
  • Kiran R Kharat
  • Arun S Kharat Department of Biotechnology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, India


The Argemone mexicana L plant, commonly found on desolate land of Marathwada region of Maharashtra state, India has been used for treating oral cavity infections. We sought to investigate the antimicrobial potential of A. Mexicana L. In this study, cold aqueous and methanolic extracts were prepared from A. mexicana L leaves. These extracts were tested for their antibacterial activities against selected bacterial isolates. The antibacterial activity and MICs were tested using the agar well diffusion method. The cold aqueous and methanolic extracts of A. mexicana L leaves inhibited growth of Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa.Antibacterial potentiality of A. mexicana L extracts was compared to that of inhibitory zones obtained Streptomycin.  Curiously, antibacterial activity of the extract was superior to streptomycin, a broad spectrum antibiotic used as the standard reference antibiotic. The active ingredient of antibacterial potentiality within the A. mexicana L extract was confirmed by TLC, HPLC and NMR analysis.  Structural elucidation of Berberine and its bioactivity both from A. mexicana L and commercial preparation is discussed.

Author Biography

Arun S Kharat, Department of Biotechnology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, India
Professor in Biotechnology


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