Developmental changes in the levels and redox potentials of main hemolymph thiols/disulfides in the Jamaican field cricket Gryllus assimilis

  • Izabela A. Sadowska-Bartosz University of Rzeszów, Department of Biochemistry and Cell Biology
  • Paulina Furmaniak University of Łódź Department Environmental Chemistry
  • Edyta Bieszczad-Bedrejczuk University of Rzeszów, Department of Biochemistry and Cell Biology
  • Grzegorz Bartosz University of Łódź Department of Molecular Biophysics
  • Rafał Głowacki University of Łódź Department Environmental Chemistry


Main thiols and disulfides were determined in the hemolymph of the Jamaican field cricket Gryllus assimilis of various developmental stages. On the basis of these data, redox potentials of the glutathione, cysteine and homocysteine redox systems were calculated. The concentrations of all thiols studied decreased during development (at a stage of 6 molts) with respect to young crickets, and increased again in adult insects. Redox potentials of the glutathione and cysteine systems increased from values of  -131.0 ± 5.6 mV and -86.9 ±17.1 mV, respectively  in young crickets to -58.0 ± 3.6 mV and -36.1 ± 4.2 mV, respectively, at the stage of 6 molts and decreased to values of -110.4 ± 24.8 mV and -66.3 ± 12.2 mV, respectively, in adult insects. Redox potentials of the glutathione and cysteine systems in the hemolymph of young and adult insects were similar to those reported for human plasma, suggesting similar redox conditions of extracellular fluids in insects and in mammals.


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