The influence of CSN3 and LGB polymorphisms on milk production and chemical composition in Romanian Simmental cattle
The aim of the study was to assess the influences of genotypes from κ-casein (CSN3) and β-lactoglobulin (LGB) loci on milk production and chemical composition in Romanian Simmental cattle. For CSN3 locus a significant increased frequency (P ≤ 0.001) was observed for the allele A (0.785) compared with allele B (0.215) and for AA (0.631) compared to AB (0.307) and BB (0.062) genotypes. The AA genotype was associated with a higher milk production (5887.76±115.7 kg) compared to the yields associated with BB genotype (5619±86.34 kg, P ≤ 0.003) and a higher fat percentage (4.19±0.05% vs. 4.01±0.02%, P ≤ 0.001). A higher milk protein percentage was associated with BB (3.4±0.02%) compared to AA (3.27±0.03) and AB (3.29±0.03) genotypes (P ≤ 0.05). For LGB locus, the frequency of allele A was significantly higher (0.606, P ≤ 0.008) than allele B (0.394). The predominately AB genotype was the most prevalent in herd (0.579) compared to AA (0.316) and BB genotypes (0.105). The AB genotype was associated with a higher milk production (5906.54±166.76 kg) compared with the other two genotypes (P ≤ 0.05). The BB genotype was associated with a higher fat percentage in milk (4.23±0.06%) compared with AA (4.17±0.06) and AB (4.2±0.08) genotypes (P ≤ 0.01). For the LGB locus no significant differences (P > 0.05) were observed for milk protein percentage. The current outcomes encourage improving genetic structure in cattle, based on marker assisted selection for genes with economic values, in order to increase the quantity and quality of milk.
Acatincai S, Cziszter LT (2010) Producțiile animalelor. Agroprint Ed., p112-115. Timișoara, Romania.
Ahmadi M, Mohammadi Y, Darmani Kuhi H, Osfoori R, Qanbari S (2008) Association of milk protein genotypes with production traits and somatic cell count of Holstein Cows. J Biol Sci, 8: 1231-1235. DOI: 10.3923/jbs.2008.1231.1235
Azevedo AL, Nascimento CS, Steinberg RS, Carvalho MR, Peixoto MG, Teodoro RL, Verneque RS, Guimaraes SE, Machado MA (2008) Genetic polymorphism of the kappa-casein gene in Brazilian cattle. Genet Mol Res 7: 623-630. DOI: 10.4238/vol7-3gmr428
Balteanu VA, Vlaic A, Rusu AR, Creanga S, Pop FD, Cighi V (2007) Milk protein polymorphism in some Romanian cattle breeds identified by isoelectric focusing technique (IEF). Sci Pap Anim Sci Series 50: 173-181.
Bencsik I, Pacala N, Stanculet J, Telea A, Bencsik A (2007) The influence of k-casein alleles on milk production and quality in a Holstein-Frisian cow population. Sci Pap Anim Sci Biotech 40: 13-17.
Deb R, Chakraborty S, Singh U (2012) Molecular markers and their application in livestock Genomic research. J Vet Sci Technol 3:e108. doi:10.4172/2157-7579.1000e108
He F, Sun DX, Yu Y (2006) Association between SNPs within prolactin gene and milk performance traits in Holstein dairy cattle. Asian Austral J Anim Sci 19: 1384-1389. doi: https://doi.org/10.5713/ajas.2006.1384
Hiendleder S, Thomsen H, Reinsch N, Bennewitz J, Leyhe-Horn B, Looft C, Xu N, Medjugorac I, Russ I, Kuhn C, Brockmann GA, Blumel J, Brenig B, Reinhardt F, Reents R, Averdunk G, Schwerin M, Forster M, Kalm E, Erhardt G (2003) Mapping of QTL for body conformation and behaviour in cattle. J Hered 94: 496-506. doi: 10.1093/jhered/esg090
Hill T, Lewicki P (2007) Statistics: Methods and Applications. StatSoft, Tulsa, OK, USA.
Ilie DE, Magdin A, Salajeanu A, Neamt RI, Vintila I (2008) Analysis of genetic polymorphism at the CSN3 and BLG locus in some dairy breeds from west part of country. Scientific reports of ICDCB Balotesti, Ceres Ed, p 40-45, Romania.
Ilie DE, Magdin A, Salajeanu A, Neamt RI, Vintila I (2009) Influence of CSN 3 marker on milk composition in Romanian Brown and Romanian Simmental cattle from SCDCB Arad. Sci Pap Anim Sci Biotech 42: 54-57.
Ilie DE, Neamt RI (2015) Effects of sires genotyping for κ-casein on B allele frequency in Romanian Brown cattle. In: International scientific symposium, Bioengineering of animal resources, p 204, Timisoara, Romania.
Kucerova J, Matejicek A, Jandurova OM, Sorensen P, Nemcova E, Stipkova M, Kott T, Bouska J, Frelich J (2006) Milk protein genes CSN1S1, CSN2, CSN3, LGB and their relation to genetic values of milk production parameters in Czech Fleckvieh. Czech J Anim Sci 51: 241-247.
Kuhn C, Bennewitz J, Reinsch N, Xu N, Thomsen H, Looft C, Brockmann CA, Schwerin M, Weimann C, Hiendleder S, Erhardt G, Medjugorac I, Förster M, Brenig B, Reinhardt F, Reents R, Russ I, Averdunk G, Blumel J, Kalm E. (2003) Quantitative trait loci mapping of functional traits in the German Holstein cattle population. J Dairy Sci 86: 360–368. doi: 10.3168/jds.S0022-0302(03)73614-5
Singh LV, Jayakumar S, Sharma A, Gupta SK, Dixit SP, Gupta N, Gupta SC (2015) Comparative screening of single nucleotide polymorphism in β-casein and k-casein gene in different breeds of India. Meta Gene 4: 85-91. doi: 10.1016/j.mgene.2015.03.005
Lara MAC, Gama LT, Bufarah G, Sereno JRB, Celegato EML, Abreu UP (2002) Genetic polymorphisms at the k -casein locus in Pantaneiro cattle. Arch Zootec 51: 99-105.
Matejicek A, Matejickova J, Nemcova E, Jandurova OM, Stipkova M, Buska J, Frelich J (2007) Joint effects of CSN3 and LGB genotypes and their relation to breeding values of milk production parameters in Czech Fleckvieh. Czech J Anim Sci 52: 83-87.
Medrano JF, Cordova EA (1990a): Genotyping of bovine kappa-casein loci following DNA sequence amplification. Biotechnology 8: 144-146.
Medrano J.F., Cordova E.A. (1990b): Polymerase chain reaction amplification of bovine LGB genomic sequences and identification of genetic variants by RFLP analysis. Anim Biotech 1: 73-77.
Ogorevc J, Kunej T, Razpet A, Dovc P (2009) Database of cattle candidate genes and genetic markers for milk production and mastitis. Animal Genetics 40: 832–851. doi: 10.1111/j.1365-2052.2009.01921.x. Epub 2009 Jun 8
Ojala M, Famula TR, Medrano JF (1997) Effects of milk production genotypes on the variation of milk production traits of Holstein and Jersey cows in California. J Dairy Sci 80: 1776-1785.
Ron M, Yoffe O, Ezra E, Mendrano JF, Well JI (1994) Determination of effects of milk protein genotype on production traits of Israeli Holsteins. J Dairy Sci 77: 1106-1113.
Shapiro SS, Wilk MB (1965) An analysis of variance test for normality (complete samples). Biometrika 52: 591–611.
Sitkowska B, Neja W, Wisniewska E (2008) Relations between kappa-casein polymorphism (CSN3) and milk performance traits in heifer cows. J Cent Eur Agric 9: 641-644.
Strzalkowska N, Josef K, Lech Z, Zofia R (2002) Effects of kappa-casein and beta-bactoglobulin loci polymorphism, cows age, stage of lactation and somatic cell count on daily milk yield and milk composition in Polish Black and White cattle. Anim Sci Pap Rep 20: 21-35.
Sulimova GE, Abani AM, Rostamzadeh I, Mohammad AMR, Lazebnyi OE (2007) Allelic polymorphism of kappa-casein gene (CSN-3) in Russian cattle breeds and its informative value as a genetic marker. Genetika 43: 88-95.
Tsiaras AM, Bargouli GG, Banos G, Boscos CM (2005) Effect of kappa-casein and beta-lactoglobulin loci on milk production traits and reproductive performance of Holstein cows. J Dairy Sci 88: 327–334. DOI: 10.3168/jds.S0022-0302(05)72692-8
Umesh S, Rajib D, Rafeeque RA (2014) Molecular markers and their applications in cattle genetic research: A review. Biomarkers and Genomic Medicine 6: 49-58. http://dx.doi.org/10.1016/j.bgm.2014.03.001
Van der Berg G, Escher JTM, De Koning PJ, Bovenhuis H (1992) Genetic polymorphism of κ-casein and β-lactoglobulin in relation to milk composition and processing. Netherland Milk Dairy J 46:145-168.
Yahya M, Ali AA, Mohammad RN, Ali EK (2013) Allelic polymorphism of κ-casein, β-lactoglobulin and leptin genes and their association with milk production traits in Iranian Holstein cattle. J Cell Mol Res 5: 75-80. DOI:10.14675/jcmr.v5i2.21086
Acta Biochimica Polonica is an OpenAccess quarterly and publishes four issues a year. All contents are distributed under the Creative Commons Attribution-ShareAlike 4.0 International (CC BY 4.0) license. Everybody may use the content following terms: Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
Copyright for all published papers © stays with the authors.
Copyright for the journal: © Polish Biochemical Society.