MMP-10, MMP-7, TIMP-1 and TIMP-2 mRNA expression in esophageal cancer

  • Agnieszka Juchniewicz Department of Clinical Molecular Biology, Medical University of Bialystok, 13 Waszyngtona Str, 15-267, Bialystok, Poland
  • Oksana Kowalczuk Department of Clinical Molecular Biology, Medical University of Bialystok, 13 Waszyngtona Str, 15-267, Bialystok, Poland
  • Robert Milewski Department of Statistic and Medical Informatics, Medical University of Bialystok, 37 Szpitalna Str, 15-295 Bialystok, Poland
  • Wojciech Laudański Department of Thoracic Surgery, Medical University of Bialystok, 24A M. Skłodowskiej-Curie Str, 15-267, Bialystok, Poland
  • Piotr Dzięgielewski Department of Thoracic Surgery, Medical University of Bialystok, 24A M. Skłodowskiej-Curie Str, 15-267, Bialystok, Poland
  • Mirosław Kozłowski Department of Thoracic Surgery, Medical University of Bialystok, 24A M. Skłodowskiej-Curie Str, 15-267, Bialystok, Poland
  • Jacek Nikliński Department of Clinical Molecular Biology, Medical University of Bialystok, 15-267 Białystok, Poland.
Keywords: Esophageal cancer, gene expression, metalloproteinases,


Introduction: Tissue inhibitors of metalloproteinases (TIMP) and the matrix metalloproteinases (MMP) are involved in the spread of cancer. Methods: We have evaluated the matrix metalloproteinases’ (MMP-10, MMP-7) and their inhibitors’ (tissue inhibitors of metalloproteinases – TIMP-1, TIMP-2) mRNA expression in 61 esophageal cancer samples from patients who had undergone surgery, by using real-time quantitative RT-PCR, and correlated the results with the patient clinicopathologic features. Results: MMP-10, MMP-7, TIMP-1, TIMP-2 were overexpressed in 73%, 85%, 55% and 42% of esophageal cancer samples, respectively. The expression of MMP-10, TIMP-1, and TIMP-2 correlated with the tumor size. The MMP-7 overexpression was associated with the tumour stage (I, II vs III, p=0.05) and lymph node metastasis (N0 vs N1, p=0.037). Conclusions: We conclude that in the resected esophageal cancer an increased mRNA expression of MMP-7, MMP-10 and TIMP-1 correlated with clinicopathologic features. We suggest that these genes may play a role during progression of the disease.


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