New functionality of RNAComposer: application to shape the axis of miR160 precursor structure

  • Maciej Antczak Institute of Computing Science, Poznan University of Technology
  • Mariusz Popenda Institute of Bioorganic Chemistry, Polish Academy of Sciences
  • Tomasz Zok Institute of Computing Science, Poznan University of Technology
  • Joanna Sarzynska Institute of Bioorganic Chemistry, Polish Academy of Sciences
  • Tomasz Ratajczak Institute of Computing Science, Poznan University of Technology
  • Katarzyna Tomczyk Institute of Computing Science, Poznan University of Technology
  • Ryszard Walenty Adamiak Institute of Bioorganic Chemistry, Polish Academy of Sciences
  • Marta Szachniuk Institute of Bioorganic Chemistry, Polish Academy of Sciences and Institute of Computing Science, Poznan University of Technology
Keywords: RNA 3D structure prediction, RNAComposer, plant miRNA precursor


RNAComposer is a fully automated, web-interfaced system for RNA 3D structure prediction, freely available at and Its main components are: manually curated database of RNA 3D structure elements, highly efficient computational engine and user-friendly web application. In this paper, we demonstrate how the latest additions to the system allow the user to significantly affect the process of 3D model composition on several computational levels. Although in general our method is based on the knowledge of secondary structure topology, currently RNAComposer offers a choice of six incorporated programs for secondary structure prediction. It allows also to apply conditional search in the database of 3D structure elements and introduce user-provided elements into the final 3D model. This new functionality contributes to a significant improvement of the predicted 3D model reliability and it facilitates better model adjustment to the experimental data. This is exemplified based on RNAComposer application for modelling of the 3D structures of precursors of miR160 family members.

Author Biography

Marta Szachniuk, Institute of Bioorganic Chemistry, Polish Academy of Sciences and Institute of Computing Science, Poznan University of Technology
Laboratory of Bioinformatics


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