Endothelium as target for large-conductance calcium-activated potassium channel openers

The endothelium is a highly active organ responsible for vasculatory tone and structure, angiogenesis, as well as hemodynamic, humoral, and inflammatory responses. The endothelium is constantly exposed to blood flow, sheer stress and tension. Endothelial cells are present as a vasculature in every tissue of the body and react to and control its microenvironment. A variety of ion channels are present in the plasma membranes of endothelial cells. These include potassium channels such as inwardly rectifying potassium (Kir) channels, voltage-dependent (Kv) channels, ATP-regulated potassium (KATP) channels and three types of calcium-activated potassium channels (KCa), the large (BKCa), intermediate (IKCa), and small (SKCa) -conductance potassium channels. Potassium current plays a critical role in action potentials in excitable cells, in setting the resting membrane potential, and in regulating neurotransmitter release. Mitochondrial isoforms of potassium channel contribute to the cytoprotection of endothelial cells. Prominent among potassium channels are families of calcium-activated potassium channels, and especially largeconductance calcium-activated potassium channels. The modulation of BKCa channels, which are voltageand calcium-dependent, has been intensively studied. The BKCa channels show large expression dynamics in endothelial cells and tissue-specific expression of large numbers of alternatively spliced isoforms. In this review, a few examples of the modulatory mechanisms and physiological consequences of the expression of BKCa channels are discussed in relation to potential targets for pharmacological intervention.


INTRODUCTION
The endothelium is a monolayer of the cells that line the entire internal surface of the blood vessels and lymphatic system.The term endothelium was introduced by the anatomist Wilhelm His in 1865, and for a long time it was considered to be an inert "layer of nucleated cellophane" serving only as a non-reactive barrier (Galley & Webster, 2004).
It is now well established that the endothelium is a very important active component of the cardiovascular system and has autocrine and paracrine activities (Galley & Webster, 2004).Endothelial cells (ECs) regulate vascular tone and blood flow, thrombosis and thrombolysis, and platelet adherence processes.The main role of the endothelium is to regulate vascular tone by releasing vasodilator and vasoconstrictor substances (Table 1).The endothelial cells in the vascular tree are not uniform in shape, thickness or expression of cell adhesion molecules (e.g., ICAM, VCAM, PECAM).The endothelium differs among the leukocyte trafficking between the skin, muscle mesentery (with classical multistep for leukocyte recruitment), and the lung, liver, and mescendrinc lymph nodes (Aird, 2007a;Dietmar, 2007;Aghajanian et al., 2008;Wittchen, 2009).Each EC is a dynamic structure that responds to the extracellular environment, which may include mechanical (e.g., shear stress and tension) or biochemical factors (e.g., cytokines, hormones, growth factors, ROS, NO).These environmental factors cause endothelial phenotypic changes which can alter as cell shape, calcium influx, protein expression, mRNA levels, migration, proliferation, apoptosis and survival, vasomotor tone, inflammatory response, leukocyte adhesion and migration.Because the endothelium is distributed through the body and has contacts with every tissue, its dys-function can influence the state of each tissue in the body (Aird, 2007b).A rapid progress in the documentation of the phenotypic heterogeneity of the endothelium with the use of different approaches has been achieved recently (e.g., immunohistochemistry, in situ hybridisation, real-time microscopy, and proteomic techniques) (Pasqualini & Arap, 2002;Aird, 2003;Shibata et al., 2005;Shin & Anderson, 2005;Sandow & Grayson, 2009).The endothelial phenotypic changes related to the environment can clearly be seen in the formation of endothelium in the blood-brain barrier, where endothelium is under the regulation of astroglial-derived paracrine factors.Another example are the ECs lining microvessels in the heart, which are exposed to the mechanical forces generated by contracting cardiomyocytes and to their paracrine and electrical factors (Hsieh et al., 2006).In embryogenesis, the mesoderm is the exclusive source of ECs precursors, which are in close colocalisation with haematopoietic precursor cells, and this has suggested that both arise from hemangioblasts.In the adult body, ECs in quiescent vasculature are proliferatively inactive with a relatively long life-span (Hobson & Denekamp, 1984;Ferran, 2006;Langenkamp & Molema, 2009).It is important to note that tumours depend on new vasculature supply for their growth, and it is crutial to characterise the phenotype of the ECs to understand the action of anti-angiogenic drugs that can affect tumours through their vasculature (Aird, 2009;Langenkamp & Molema, 2009).The endothelium is in constant balance between vasodilatation and vasoconstriction, proliferation and its inhibition, activation of smooth muscle cell migration, and activation and inhibition of adhesion, thrombogenesis and fibrinolysis.Shifts in the metabolism of the endothelium toward reduced vasodilatation, a proinflammatory state, and prothrombic characteristics lead to endothelial dysfunction (Feletou & Vanhoutte, 2006a;Vanhoutte et al., 2009).The actions of endothelial vasoactive components very often involve the activation of potassium channels, especially calcium-activated potassium channels (K Ca ), a key component in the regulation of membrane potential in endothelial and smooth mus- cle cells that are among the regulatory components of vascular tone (Nelson & Quayle, 1995;Nilius & Droogmans, 2001).The endothelium-dependent response to aggregating platelets is not present to the same extent in all arteries, but is most prominent in the coronary and cerebral circulation.When analysing the architecture of the vasculature, the internal elastic lamina (IEL), with the fenestration required for myoendothelial gap junctions (MEGJ), is worthy of note, as it is probably related to the presence of sites of low resistance passage for the diffusion-mediated release of vasoactive endothelial and smooth muscle substances.The MEGJ are specialised structures with microdomains representing a selective target for the control of endothelial and vascular functions (Sandow et al., 2009a;2009b).The most important component of the control of vascular tone is regulation by potassium channels, which are themselves regulated by the best known endothelial releasing factors nitric oxide (NO) and prostacyclin (PGI 2 ).There are also other regulatory factors released from endothelium, known as endothelium-derived hyperpolarising factors (EDHF), which are not fully characterised and are associated with hyperpolarisation of the underlying endothelium smooth muscle cells (Feletou & Vanhoutte, 2006b).
A variety of ion channels are present in the plasma membranes of endothelial cells.These include potassium channels such as Ca 2+ -activated K + channels (BK Ca channels), inwardly rectifying K + channels (K IR channels), and voltage-dependent K + channels (K V channels).Endothelial potassium channels have been implicated in endothelium-dependent vasodilation.Setting the membrane potential (V m ) leads to modulation of endothelial Ca 2+ signalling and the synthesis of vasodilating factors.Different levels of potassium ion channel expression and a variety of alternative splicing particularly in variants of BK Ca channels have multiple interactions with tissue-specific proteins and a large diversity of interactions with the microenvironments of ECs in the vasculature.Expression of specific channels responsible for stabilisation of the resting membrane potential and its changes are the paramount task for specific parts of endothelium (Nilius et al., 1997;Nilius & Droogmans, 2001;Schmidt et al., 2008).

ENDOTHELIAL POTASSIUM CHANNELS
Although ECs are not electrically excitable, a large number of the signalling functions performed by the vascular endothelium depend on the modulation of activity of endothelial cell ion channels.ECs secrete a variety of endothelium-derived vasoactive molecules and endothelium-derived hyperpolarising factors (EDHF) required for rapid calcium entry (Carter et al., 1988;Lantoine et al., 1998).Additionally, gap junction proteins (connexins) functionally couple ECs in an electrical fashion in some specific regions to smooth muscle cells.These connections permit the spreading of changes in membrane potential (V m ) in ECs to the underlying excitable tissue (De Wit et al., 2006;De Wit & Wolfle, 2007).Changes in ECs membrane potential occur in response to a variety of stimuli (e.g., shear stress, hypertension, cytokines) (Mehrke & Daut, 1990;Barakat et al., 1999;Chauhan et al., 2003).The nature of the intracellular calcium dynamics and signalling in the ECs of the native endothelium are still unclear in comparison to the vascular myocytes (Tran & Watanabe, 2006).Potassium channels are the most diverse class of ion channels underlying electrical signalling in the cell, especially in excitable cells where they play a fundamental role in the regulation of action potential (AP).Potassium channels are ion-selective cation channels with an equilibrium potential near the typical potential of resting cells.A multiplicity of ion channels are present in the plasma membranes of ECs, including inwardly rectifying potassium (K ir ) channels, voltage-dependent (K v ) channels and ATPregulated potassium (K ATP ) channels and a group of channels also responsible for modulation of the membrane potential in endothelial cells are Ca 2+ -activated K + channels (K Ca channels) (Nilius & Droogmans, 2001;Taylor et al., 2003).

Endothelial calcium-activated potassium channels (K Ca )
Elevation of intracellular calcium concentration [Ca 2+ ] i in ECs is the first response to most stimuli experienced by the cell.The Ca 2+ influx into the ECs depends on the electrochemical gradient set primarily by membrane potential.Influx of Ca 2+ into the ECs leads to depolarisation of the membrane, which is compensated by the activation of K Ca channels.An increased opening probability of K Ca at elevated [Ca 2+ ] i causes ECs membrane hyperpolarisation and a driving force for Ca 2+ entry through the opened calcium channels.Three types of calciumactivated potassium channels (K Ca ), the large (BK Ca ), intermediate (IK Ca ), and small (SK Ca ) conductance potassium channels, have been identified in the vascular wall (Table 2).

LArgE-CONDUCTANCE CALCIUM-ACTIvATED POTASSIUM CHANNELS (BK CA )
The discovery in many tissues of a large outward K + current with a dependence on calcium influx and membrane depolarisation has led to the identification of large conductance calcium-activated potassium (BK Ca ) channels (Heyer & Lux, 1976;Gorman & Thomas, 1980;Pallotta et al., 1981).A mammalian ortholog of Drosophila slo, Slo1, was cloned by hybridisation of a mammalian cDNA library using the Drosophila 'slowpoke' (slo) cDNA (Pallanck & Ganetzky, 1994).The BK Ca channel encoded by the Slo1 gene (KCNMA1) is expressed in many excitable and nonexcitable cells.BK Ca channels play a role in the control of vascular tone, coupling local increases in intracellular Ca 2+ to membrane hyperpolarisation and vascular relaxation.BK Ca channels can be activated by membrane depolarisation or intracellular calcium [Ca 2+ ] i separately or by both factors synergistically (Magleby, 2003).The BK Ca channel belongs to the group of six/seven-transmembrane potassiumselective channels and consists of four α-and four auxiliary β-subunits (Knaus et al., 1994a;Tanaka et al., 1997).The pore-forming α subunit is encoded by the KCNMA1 gene, which produces multiple isoforms through alternative splicing.The KCNMA1 gene is located in the chromosome region 10q22.3(Pallanck & Ganetzky, 1994;Du et al., 2005).The α subunit and four β (1-4) subunits are encoded by different genes that show tissue-specific expression (Higgins et al., 2008;Latorre & Brauchi, 2006;Sausbier et al., 2004;Yu et al., 2006).Different combinations of the β-subunits with α-subunit splice variants generate a physiologically diverse complement of BK Ca channels that differ dramatically in their tissue distribution, trafficking, and regulation (e.g.individual splice variants are differentially sensitive to phosphorylation by cAMP-dependent protein kinase), whose parameters provide the kinetic range needed for electrical fine-tuning (Chen et al., 2005;Langer et al., 2003;Ma et al., 2007;Ramanathan et al., 1999).For a long time the expression of BK Ca channels in ECs was questioned (Nilius & Droogmans, 2001).Currently, however, it is accepted that ECs express BK Ca channels at the mRNA and protein levels (Haburcak et al., 1997;Chiang & Wu, 2001;Wang et al., 2005;Dong et al., 2007).It has also been shown that the BK Ca channel opener CGS7184 can cause endothelium-dependent vasodilatation in isolated aorta rings in a dose-dependent manner, increase NO production, and influence on mitochondrial membrane potential in the endothelial cell line EA.hy 926 (Wrzosek et al., 2009).The discrepancies regarding the existence of BK Ca channels in ECs were likely caused by the tremendous diversity of ECs along the blood arteries and differences in the preparations obtained for studies of BK Ca channel expression and function from freshly isolated and cultured vascular ECs, which are known to exhibit phenotypic drift.There is evidence for the presence of endothelial BK Ca channels that have a potential for rapid upregulation in some intact vessels, which may occur in disease (Sandow & Grayson, 2009).Because the BK Ca  (Kamouchi et al., 1997).Studies have shown that polymorphism of the β 1 regulatory subunit of the BK Ca channel modulates the risk of diastolic hypertension in humans.

Modulation of BK Ca channel activity by ROS and RNS
It is well documented that reactive oxygen (ROS) and reactive nitrogen (RNS) species are produced in ECs, and they are very important factors in controlling the cardiovascular function (Droge, 2002;Gutterman et al., 2005;Pacher et al., 2007;Wolin, 2009).The endothelium can generate ROS and RNS through the enzymatic activity of nitric oxide synthases (NOS) (i.e., endothelial NOS (eNOS or NOS3 or cNOS) and inducible NOS (iNOS or NOS2)), xanthine oxidases, NAD(P)H oxidases, cyclooxygenases, cytochrome P450-dependent oxygenases, and leakage of electrons from mitochondria to generate superoxide (O 2 •-) (Basuroy et al., 2009;Gutterman et al., 2005;Turrens, 2003).NO (also known earlier as endothelium-derived relaxing factor (EDRF)) was discovered as a compound that causes vascular smooth muscle relaxation in the presence of endothelium after stimulation by acetylcholine (ACh) (Furchgott & Zawadzki, 1980).Endothelium-derived RNS and ROS have been proposed to regulate vascular tone via complex mechanisms, one of them being the modulation of BK Ca channel function (Matalon et al., 2003).Most information regarding the actions of NO on BK Ca channels comes from studies of vascular smooth muscle preparations.The relaxation caused by NO and NO donors (e.g., NTG, NONOate, SIN-1) and prostacyclin (PGI 2 ) and its synthetic analogues (e.g., beraprost, iloprost, cicaprost) is associated with concomitant hyperpolarisation of smooth muscle cells (Tanaka et al., 2004).The important features of NO cellular actions are its high membrane permeability and short half-life, which is in the range of seconds.Other free radicals, metal-containing proteins, thiols, and oxygen are the major targets for NO.The NO released from ECs and many nitrovasodilators (e.g., nitroglycerine (NTG)) has been proposed to mediate smooth muscle relaxation via the stimulation of soluble guanylate cyclase (sGC) (Gruetter et al., 1981;Cayabyab & Daniel, 1995).It is also well documented that muscle relaxation and membrane hyperpolarisation in smooth muscle can be induced by released NO in a manner independent of cyclic guanosine monophosphate (cGMP) (Bolotina et al., 1994;Watson et al., 1996).At least three possible mechanisms by which NO activates BK Ca channels and leads to vascular smooth muscle relaxation have been proposed, including direct activation by NO via modulation of -SH groups, phosphorylation of the BK Ca channel by cGMP-dependent protein kinase (PKG), and inhibition of NO formation by 20hydroxyeicosatetraenoic acid (20-HETE), an inhibitor of BK Ca channel activity.It is also possible that NO can modulate proteins that interact in vivo with BK Ca channels.NO was shown to activate BK Ca channels in a cGMP-independent manner via a direct modification of BK Ca channels from vascular smooth muscle (Bolotina et al., 1994;Abderrahmane et al., 1998;Mistry & Garland, 1998;Ahern et al., 1999;Lang et al., 2000).Direct modulation of BK Ca channel activity by NO and ROS has been demonstrated in renal artery endothelium (Brakemeier et al., 2003).Those authors identified BK Ca channels in the endothelium of porcine renal arteries using the patch-clamp technique in situ.The activity of the channel was controlled by calcium concentration and membrane potential and was inhibited by Ba 2+ and iberiotoxin (IbTx), a potent and specific blocker of BK Ca channels.NO donors also activated the channel.It is interesting that hydrogen peroxide led to a dosedependent inactivation of BK Ca and caused inhibition of vasodilatation of isolated porcine artery after bradykinin treatment.It has been shown that in contrast to NO, intracellular and extracellular challenge of endothelial BK Ca channels with H 2 O 2 and ROS results in a dose-dependent and irreversible channel inactivation (Brakemeier et al., 2003).Such an inhibition by H 2 O 2 has also been reported for another type of K Ca channel, the intermediate-conductance K Ca (IK Ca ), in bovine aortic ECs (Cai & Sauve, 1997).Thus, it is likely that other intracellular second messengers in addition to [Ca 2+ ] i co-stimulate endothelial BK Ca channel activity.Therefore, such a stimulatory effect on whole-cell currents through BK Ca channels might be a result of such an H 2 O 2 -induced influx of Ca 2+ , which presumably overrides the direct inhibitory effects of H 2 O 2 on the channel activity (Gupta et al., 2001).There are also studies that support the hypothesis that NO cannot directly modulate BK Ca channel activity.It was shown using whole cell and patch-clamp techniques, that BK Ca channels are present in the endothelial cell line EA.hy 926 and are not stimulated directly by NO (Haburcak et al., 1997).Hydrogen peroxide is produced in endothelial and smooth muscle cells from O 2 •-, primarily enzymatically by superoxide dismutase.As previously mentioned, H 2 O 2 can act as a vasoconstrictor or, depending on the tissue and the experimental conditions, can have dilatory properties that lead to hyperpolarisation of the vascular smooth muscle membrane (Ellis & Triggle, 2003).Oxidative stress is

2009
A. Wrzosek also recognised as a significant determinant of BK Ca channel function (DiChiara & Reinhart, 1997;Wang et al., 1997;Liu & Gutterman, 2002).It has been proposed that the mechanisms of these changes involve the oxidation of cysteine residues located in the intracellular and C-terminal regions of the channel that alter its voltage-and calcium-dependence (DiChiara & Reinhart, 1995).
Moreover, it has been documented that both redox modulation and nitrothiosylation of cysteine residues on the cytosolic surface of the BK Ca channel protein can alter channel gating (Lang et al., 2000).

Modulation of BK Cs channel activity by carbon monoxide
Carbon monoxide is an endogenous gaseous messenger that regulates physiological function in a variety of tissues in a paracrine and autocrine manner.CO is a deadly poisonous gas physiologically produced during heme catabolism by heme oxygenases (HOs) and is recognised as a biological signalling molecule (Jaggar et al., 2005;Abraham & Kappas, 2008).CO is important in the regulation of vascular tone, synaptic plasticity, and tumour proliferation (Kim et al., 2006).HO-1 and CO play roles in various aspects of vascular disorders, cancer, vascular restenosis, hypertension-impaired wound healing, ischemia/reperfusion, peripheral vascular disease, and atherosclerosis (True et al., 2007;Abraham & Kappas, 2008;Dulak et al., 2008).One target of CO modulation is ECs, where it can modulate the BK Ca channel directly as well as via a mechanism involving NO or the cGMP-dependent pathway (Dong et al., 2007).BK Ca channels are involved in the hypoxia-signalling cascade of a number of cellular systems.It has been shown that knockdown of HO-2 expression leads to a reduction in BK Ca channel activity, and the CO production by HO-2 reduces this loss of function (Williams et al., 2004).Specificity to hypoxia is conferred by a highly conserved motif in the stress-regulated exon (STREX) of the BK Ca channel α-subunit splice variant.Expression of the STREX splice variant is tissue-specific and can provide the control mechanism for cellular responses to hypoxia.Mutation of the serine (S24) residue abolished the hypoxia sensitivity of the STREX splice variant (Mc-Cartney et al., 2005).Recently, a structural motif that acts as a sensor of CO was localized to the C-terminal tail of the human BK Ca channel within the RCK1 domain and a high-affinity Ca 2+ sensor (Hou et al., 2008;Williams et al., 2008).In BK Ca channels, motifs that bind reduced heme have been recognised.The data support the hypothesis that reduced heme is a functional CO receptor for BK Ca channels and could provide a mechanism by which gaseous messengers regulate the channel activity (Jaggar et al., 2005).In fact, CO-mediated activation of BK Ca channels can participate in the mesenteric arterial vasodilatation of ascetic cirrhotic rats (Bolognesi et al., 2007).It has also been documented that CO and biliverdin can prevent endothelial cell sloughing in diabetic rats, probably by decreasing oxidative stress (Rodella et al., 2006).The role of the CO and HO-2 pathway in astrocyte signalling is to activate BK Ca channels in smooth muscle arterioles and dilate them (Li et al., 2008a).Recently, it was shown that ECs respond to sheer stress by producing a sustained increase in NO, and a transient increase in ROS production can activate the HO-1 gene.This process is regulated by mitochondria-derived H 2 O 2 that diffuses into the cytosol, leading to HO-1 up-regulation and maintenance of ECs protection (Li et al., 2008b).The protective role of CO was demonstrated using the tricarbonylchloro(glycinato)ruthenium (II) (CORM-3) CO carrier in mice with lethal sepsis.Delivery of a controlled amount of CO dramatically reduced mortality in septic mice by supporting mitochondrial energetic metabolism (Lancel et al., 2009).Variuos CO-releasing molecules have been tested for their potency in cell-protective mechanisms (Masini et al., 2008;De Backer et al., 2009).The protective role of CO against hypoxia could, at least in part, be related to activation of BK Ca channels located in the plasma or inner mitochondrial membranes.role of the auxiliary β subunits in BK Ca activation BK Ca channels are accompanied by four types of regulatory auxiliary β-subunits, β1-β4, which are 191 to 235 amino-acid residues long (Knaus et al., 1994b;Wang et al., 2002).The β-subunits have two putative transmembrane segments and an extracellular loop that contains glycosylation sites and cysteine residues capable of forming disulfide bonds.The N-and C-termini of the β-subunits are oriented intracellularly.In mammals, the β-subunits are encoded by the genes KCNMB1-4 (Brenner et al., 2000;Orio et al., 2002;Liu et al., 2008).Alternative splicing of transcripts encoding the β-subunits, especially the β3-subunit, leads to expression of a large number of proteins that modify cellular function.It seems that β-subunits are not uniformly expressed in every tissue in the body, but their expression is very precisely regulated (Torres et al., 2007).It is especially remarkable that ECs do not express the regulatory βsubunit at the mRNA and protein levels (McManus et al., 1995;Tanaka et al., 1997;Papassotiriou et al., 2000), while the α-subunit of the channel is fully expressed in ECs (Kamouchi et al., 1997;Brakemeier et al., 2003).The BK Ca channel β4-subunit is preferentially localized to brain neurons, not only in the plasma membrane, but also in the inner mitochondrial membrane (Torres et al., 2007;Piwonska et al., Endothelial potassium channels 2008).It has been shown that the β4-subunit of BK Ca channels has a role in charibtotoxin (ChTx) and iberiotoxin (IbTx) resistance (Meera et al., 2000;Gan et al., 2008).It seems that the main role of β-subunits is the regulation of sensitivity to [Ca 2+ ] i and membrane potential.It was shown that β-subunits also have a protective role against digestion of BK Ca channels by trypsin, and the N-termini of the auxiliary β2subunit causes inactivation of the channel through its pore-blocking position (Zhang et al., 2009).Mice with deleted genes for β1-subunits show impairment in endothelium-dependent smooth muscle relaxation and are characterised by increased vascular superoxide production, which is probably caused by expression of vascular NADPH oxidase and leads to a reduction in cGMP-dependent kinase activity (Oelze et al., 2006).This also enhances the oxidative regulation of BK Ca channels and considerably alters the physiological voltage range at lower [Ca 2+ ] i .Those authors have shown that the M177 β1-subunit is crucial for channel activation and oxidative sensitivity (Santarelli et al., 2004).The β1-subunit enhances the internalisation of the α-subunit of the channel (Toro et al., 2006) as well as the β-subunit via endocytic trafficking signals that can regulate surface expression of the BK Ca channel (Zarei et al., 2007).The Glu65Lys polymorphism of β1-subunit is associated with reduced systolic blood pressure in middle-aged men (Nielsen et al., 2008).The BK Ca channel is responsible for ethanol tolerance at the molecular and behavioural levels (Martin et al., 2008).It was shown that β-subunit-specific modulation of BK Ca channels and their different distribution in the brain can contribute to the pathophysiologies of epilepsy and dyskinesia (Lee & Cui, 2009).These differences in distribution and expression in different tissues can be critical for the development of β-subunit-selective drugs, as has been shown (Morimoto et al., 2007).Those authors presented data demonstrating drug specificity for the β1-and β4-subunits of BK Ca channels, but not for the β2-subunit.

Proteins interacting with the BK Ca channels
There have been many observations suggesting that a large number of proteins can interact with and modulate BK Ca channel activity.Recently, studies by Kathiresan et al. (2009) using coimmunoprecipitation and 2-dimensional PAGE combined with mass spectrometry have revealed 174 putative BK Ca channel-associated proteins (BKAP) from the cytoplasmic and membrane/cytoskeletal fractions of mouse cochlea.The data revealed that 50% of these proteins have affiliations with potassium and calcium channels.It is very interesting that about 20% of the proteins are related to mitochondria.Compartmentalisation of BK Ca channels to the mi-tochondria has been found to be splice variant-specific for the BK Ca -DEC channel isoform cloned from cochlea.Those authors have identified novel BK Ca channel complexes with important roles in development, calcium binding, chaperone activity and hearing loss.The presented observations also support earlier studies that revealed a wide range of interacting proteins with cellular localisations that regulate BK Ca channel activity.Caveolae are membrane microstructures to which BK Ca channels were found to localise in bovine aortic endothelial cells (Wang et al., 2005).Caveolin-1 interacts directly with BK Ca channels and exerts a negative regulatory effect on their function.Under control conditions, it was shown that BK Ca channels could be activated by cholesterol depletion (Wang et al., 2005).In HEK293T cells, BK Ca channels have a caveolin binding motif that facilitates tethering of the channels to the membrane (Alioua et al., 2008).A possible link between BK Ca channels and the inositol 1,4,5-trisphosphate receptor (IP 3 R) via lipids rafts in the membrane has also been shown (Weaver et al., 2007).Data presented by those authors suggests a preferential association of BK Ca channels with the lipid raft domain and provides evidence for a novel structure coupling to the source of calcium.Another well-documented interaction was observed between BK Ca and IK Ca channels co-localised in membranes rich in cholesterol (Romanenko et al., 2009).These two channels work in tandem, where the IK Ca channel plays a role as a modulator for the BK Ca channel because of its higher Ca 2+ sensitivity.Membrane depletion of cholesterol disturbed the interactions between the BK Ca and IK Ca channels, which was restored by disruption of the actin cytoskeleton.In fact, an actin binding domain (ABD) were identified in BK Ca channels, and an interaction between BK Ca and actin is necessary for trafficking of BK Ca channels to the plasma membrane.This interaction is different from that with F-actin that is responsible for stretch-sensitive gating (Zou et al., 2008;Romanenko et al., 2009).LDL, and especially the oxidised form oxo-LDL, can change not only the glycocalyx, but can also modulate BK Ca channel activity.It is possible that oxo-LDL can remove cholesterol from the plasma membrane and thus modulate BK Ca channels.In the rat brain, BK Ca channels were co-purified with voltage-gated calcium channels of the L-type, P/Q-type, and N-type as macromolecular complexes.Complex formation in neurons with different types of voltage-gated calcium channels allows for rapid responses by mediating membrane hyperpolarisation that controls the neuronal release of hormones and neurotransmitters and firing patterns in the central nervous system (Berkefeld et al., 2006).In EA.hy 926 endothelial cells, the existence of BK Ca channel complexes with subplasmalemmal A. Wrzosek endoplasmic reticulum (the concept of subplasmalemmal control units (SCCU)) has been detected.These structures are responsible for local activation of BK Ca channels through the release of Ca 2+ into a limited space, leading to an increase in the local concentration of calcium ions (Frieden & Graier, 2000;Frieden et al., 2002).In many cell types, BK Ca channels are co-expressed with canonical transient receptor potential channels (TRPCs).In podocytes and human embryonic kidney (HEK293T) cells, TRPC6 and TRPC3 channels bind to BK Ca channels, and this microorganisation can serve as an increased source of Ca 2+ for the activation of BK Ca channels.Additionally, TRPC6 channels can regulate the surface expression of a subset of podocyte BK Ca channels (Larsen et al., 2007;Kim et al., 2009).Experiments employing a yeast two-hybrid screen to identify proteins that interact with BK Ca channels have detected an essential adhesion and scaffolding molecule called nephrin.From the presented data, it was suggested that nephrin plays a role in organising the surface expression of ion channel proteins in podocytes, and may be involved in outside-in signalling to adapt stimuli from neighbouring cells (Kim et al., 2008).In addition to β-subunits, Mink and the Mink-Related peptides 3, which play a role in the human heart, can directly modulate channels (Levy et al., 2008).Nordilysin convertase, a Zn 2+dependent metalloprotease, interacts in human myometrium with a specific BK Ca channel splice variant with a 44 amino-acid insertion (mK44), and is part of the molecular mechanism that regulates the excitability of smooth muscle cells (Korovkina et al., 2009).Modulation of BK Ca channel activity and direct binding have been shown for receptor of activated C kinase 1 (RACK1).RACK1 was discovered as a PKC target, and recent studies suggest that this protein acts as a scaffolding protein (Isacson et al., 2007).In addition to proteins, metabolites of arachidonic acid and other lipids can act as endothelium-derived hyperpolarising factors, which makes the regulatory picture for BK Ca channels very complex (Denson et al., 2006;Campbell & Falck, 2007;Medhora et al., 2008;Vaithianathan et al., 2008;Dhanasekaran et al., 2009).

FINAL rEMArKS
The endothelium along the vasculature displays different patterns of adhesion molecule expression and different patterns of leukocyte (macrophage) penetration.The three-dimensional organisations of the vessel and the lining ECs are also varied along the vasculature (Aird, 2007a;2007b).There is a large variety of ECs along the vascular bed that leads to different expression patterns of different iso-forms of BK Ca channels.A number of BK Ca channel isoforms in ECs are expressed only during diseased endothelial states.Considerable data exists supporting the contributions of the BK Ca channel to the development and growth of cancer, and researchers still lack highly specific modulators of this channel.The molecular heterogeneity of normal endothelium and tumour endothelium might represent an opportunity to identify specific and high-potency modulators of specific isoforms of the channel (Kunzelmann, 2005;Aird, 2009).Thus, the endothelium may constitute an attractive target for potassium channel openers that act on BK Ca channels in the plasma membrane, or even as a specific compound that can act on BK Ca channels in the mitochondrial inner membrane.Currently, a large number of potent BK Ca channel modulators are available (see: Wu et al., 2006;Bentzen et al., 2007;Nardi & Olesen, 2008;and Table 2) and future experiments showing their influence on ECs function are needed.

Table 2 . Calcium-activated potassium channels and its modulators
have a high conductance, it is conceivable that they could play an important role in the generation of membrane potential in ECs when BK Ca channels are activated and intracellular Ca 2+ is elevated.Indeed, it has been shown that the expression of BK Ca channels in cultured ECs causes a transient hyperpolarisation induced by ATP channels